Pyrazomycin and process for production thereof

ABSTRACT

Pyrazomycin is a new antibiotic produced by the fermentation of Streptomyces candidus NRRL 3601, having antiviral and antifungal activity.

United States Patent {151 3,674,774

Williams et al. July 4, 1972 PYRAZOMYCIN AND PROCESS FOR ems Cited PRODUCTION THEREOF v OTHER PUBLICATIONS [72] Inventors: Robert ll. Williams; Marvin M. l-loehn, Whaley et al., (I) Chem. Abst.," Vol. 63, 1965, p. 4093 C both of In an p i lfld- Whaley et al., (ll) Chem. Abst., Vol. 68. 1968, p. I l3353q 73 A EI'L'II ndC ,ld' l',ld.

[ 1 Sslgnee I ya ompany n lanapo n Primary Examiner--Lewis Gotts [22] Flled: 1969 Assistant Examiner-Johnnie R. Brown 2 APPL 1 7 Anomey-Everet F. Smith [52] US. Cl. ..260/210 AB, 195/80, 424/180 [57] ABSTRACT [51] Int. Cl. ..C07d 51/52 Pyrazomycin is a new antibiotic produced by the fermentation [58] Field of Search ..260/2i L5, 21 1.5 R, 210 AB f Streptomyces candidug NRRL 360i, having antiviral and antifungal activity.

1 Claim, 1 Drawing Figure PYRAZOMYCIN AND PROCESS FOR PRODUCTION THEREOF SUMMARY DESCRIPTION OF PREFERRED EMBODIMENTS Pyrazomycin (4-hydroxy-5( 3 )-ribofuranosylpyrazole-3(5 carboxamide), also arbitrarily designated by the name antibiotic A238 13, is a new antibiotic produced by the fermentation of Streptomyces candidus NRRL 3601.

When crystallized in water, pyrazomycin is obtained as colorless needles, m.p., l08-113 C. Pyrazomycin is soluble in water, methanol, ethanol, propanol, and butanol, slightly soluble in ethyl acetate, and insoluble in hydrocarbon solvents and ethyl ether. The specific optical rotation [ah of crystalline pyrazomycin, dried at room temperature in vacuo over anhydrous calcium chloride for about 15 hours, was found to be 45.5 (C 0.78 percent, w.v. (mg/ml.) in water).

Electrometric titration of pyrazomycin in a water solution revealed the presence of one titratable group of pKa 6.9.

The molecular weight of pyrazomycin, determined by mas spectral data, is 259.

The empirical formula of pyrazomycin is C,l-l N,O

Elemental analysis of pyrazomycin gave the following values:

Element Percent Theoretical Found Carbon 41.70 41.56 Hydrogen 5.00 5.25 Nitrogen 16.20 16.02 Oxygen 37.10 36.25

Pyra zoinycin is best represented by the structural formula:

- 9.30; 9.80; 11.20; 11.70; 12.90; 13.60; and 14.00 microns.

The ultraviolet spectrum of pyrazomycin obtained in acidic and neutral ethanol shows the following maxima: 232 my. (6 7.4 X 10 and 263 mp. (e 6.2 X 10 There are minima at 213 and 242 me. On addition of base, the spectrum shifts to maxima of 235 mp. (e= 5.1 X 10 and 307 mp. (e 5.1 X and minimum of 257 mp.

A powder X-ray diffraction pattern of pyrazomycin, using unfiltered chromium radiation and a wave length of 2.2896 A in calculating the interplanar spacings, gives the following values:

"d" l/I.

Pyrazomycin exhibits antifungal activity against Neumspora spp. and hence is useful, when incorporated into scrub solutions, and the like, for maintaining hospital surfaces, swimming pool areas, and the like, free of such fungal contamination.

Pyrazomycin is active in vivo against vaccinia and Herpes simplex, and is useful in treating viral infections such as small pox and cold sores at doses of from 0.5 to 250 mgJkg. daily. Pyrazomycin can be administered either orally or parenterally to warm-blooded mammals, such as mice, rats, dogs, and the like.

The in vitro activity of the above compounds against virus growth in tissue culture has been demonstrated against a number of viruses, including measles virus, Herpes simplex, vaccinia, and Coxsacki.

The ability of pyrazomycin to control the virus growth in vitro is readily demonstrated by using a plaque suppression test similar to those described by Siminofl", Applied Microbiology, 9 (1) 66-72 (1961), and DeLong,D.C. et al., Biological Evaluation of A10598, Annual of N.Y. Academy of Science 130, 44-48 (1965).

In the antiviral evaluation, plaques are seen in those areas of the plate where the virus has infected and reproduced in the cells. Zones of toxicity are also observed and the diameter thereof measured in millimeters when the test compound has a lethal cytological effect on host cells under and around the filter paper disc. Antiviral activity of the test compound is detected by observing the absence of plaques and a heavier growth of cells in a zone under and around a filter paper disc, the diameter of this zone being measured in millimeters.

The cells in a zone of activity are examined with a microscope to detennine the presence and degree of drug and/or virus damage. The staining is graded 1+, 2+, 3+, 4+, and negative to reflect the following:

4+. Dark stained areas which, upon microscopic examination, show healthy cells with no visible virus or drug damage,

3+. Less darkly stained areas that show no virus or drug damage but appear less healthy;

2+. Areas showing healthy cells with a moderate amount of virus breakthrough;

1+. Areas showing healthy cells with a greater virus breakthrough;

. No viable cells.

Table l which follows sets forth the results of the testing of pyrazomycin salts against measles virus (Edmondston strain), vaccinia (VI Lindeman), Coxsacki, and Herpes simplex. In the table, column 1 gives the concentration in terms of meg/ml. at which pyrazomycin was applied to the filter paper discs; column 2, the diameter in millimeters of the zone of virus inhibition by the test compound; column 3, the grading of stained areas; and column 4, the name'of the virus against which the compound was tested.

TABLE 1 IN VITRO ANTIVIRAL ACTIVITY OF PYRAZOMYCIN Conc. Zone Microscopic mgc./m1. mm. Examination Virus 1000 60 4+ Vaccinia 500 50 4+ Vaccinia 250 50 4+ Vaccinia 125 50 4+ Vaccinia 62 50 4+ Vaccinia 31 40 4+ Vaccinia 15 25 4+ Vaccinia 7 22 4+ Vaccinia 3.5 20 4+ Vaccinia 1000 28 3+ Herpes Simplex 500 35 3+ Herpes Simplex 250 33 3+ Herpes Simplex 125 30 3+ Herpes Simplex 62 30 3+ Herpes Simplex 31 25 3+ Herpes Simplex 15 22 2+ Herpes Simplex 7 12 2+ Herpes Simplex 3.5 10 2+ Herpes Simplex 1000 50 4+ Measles 500 43 4+ Measles 250 43 4+ Measles 125 37 4+ Measles 62 30 4+ Measles 31 37 4+ Measles 15 30 4+ Measles 7 22 4+ Measles 3.5 20 4+ Measles 1.5 15 4+ Measles 0.75 11 4+ Measles 1000 33 2+ Coxsacki 500 35 2+ Coxsacki 250 33 2+ Coxsacki 125 30 2+ Coxsacki 62 30 2+ Coxsacki 31 30 1+ Coxsacki l5 15 1+ Coxsacki 7 15 1+ Coxsacki 3.5 11 1+ Coxsacki Pyrazomycin can be produced by culturing a hitherto unknown strain of Streptomyces candidus under aerobic conditions in a culture medium containing assimilable sources of carbon, nitrogen, and inorganic salts. The organisms were first isolated from soil samples obtained from India.

The organisms were isolated from the above soil samples by suspending portions of the soil samples in sterile distilled water, and by streaking the suspensions on nutrient agar plates. The seeded nutrient agar plates were incubated at about 30 C. for several days. At the end of the incubation time, colonies of the pyrazomycin-producing organisms were transferred by means of a sterile platinum loop to agar slants. The inoculated agar slants were then incubated to provide larger amounts of inoculum for the production of pyrazomycm.

The novel organism capable of producing pyrazomycin has been placed on permanent deposit, without restriction, with the culture collection of the Northern Utilization Research and Development Division, Agricultural Research Service, US. Department of Agriculture (Formerly Northern Regional Research Laboratories), Peoria, Illinois, and is available to the public under culture No. NRRL 3601.

Because of the uncertainty of taxonomic studies with the Streptomyces group of organisms, there is always an element of doubt associated with the classification of a newly discovered organism. However, the organism which produces pyrazomycin appears to resemble most nearly, in its most important characteristics, the published description of the organism Streptomyces candzkius (Krassilnikov) Waksman on the basis of a published description, Waksman, The Actinomycetes Vol. 2 Classification, Identification and Description of Genera and Species. The Williams and Wilkins Co., Baltimore, Maryland 1963).

Culture NRRL 3601 is assigned to the Rectus-Flexibilis section, White series of Pridham et a1. (Pridham et al., "A Guide for the Classification of Streptomycetes According to Selected Groups, Applied Microbiol. 6: 52-79 (1958)), and also to either the white or yellow series of the Tresner and Backus system (Tresner and Backus, System of Color Wheels for Streptomycete Taxonomy." Applied Microbiol. 11: 335-338 (1963)). As stated above, the pyrazomycin-producing organism has been classified as a strain of Streptomyces candidus. It should be noted, however, that other species, namely Streptomyces alboflavus and Streptomyces Iongzlssimus are also similar to Streptomyces candidus NRRL 3601.

Generally speaking, Streptomyces candidus NRRL 3601 produces flexuous sporophores and cylindrical, smooth spores which are from white to pale yellow en masse. The vegetative mycelium is yellow to orange-yellow. A light yellow to light brown soluble pigment is present in two media and absent in all other media. Fascicles of aerial mycelium and sclerotia-like bodies occur on most media.

The methods employed in the taxonomic studies of the pyrazomycin-producing strain of S. candidm were those recommended for the lntemational Streptomyces Project described by Shirling and Gottlieb, Methods for Characterization of Streptomyces Species," Intent. Bull. Systematic Bauer-01.: 16, 313-340 (1966), along with other supplementary tests. Carbon utilization tests were carried out according to the method described by Pridham and Gottlieb, J. Bac. 56: 107 1948). The data obtained from the taxonomic studies are shown in tabular form below. Color names were assigned according to the lSCC-NBS method (Kelly and Judd, The lSCC-NBS Method of Designating Colors and a Dictionary of Color Names. US Dept. of Commerce Circ. 553. Washington, D.C. Figures in parentheses refer to Tresner and Backus color series (Tresner and Backus, System of Color Wheels for Streptomyces Taxonomy," Appld. Microbiol. 1 1: 335-338 (1966)). Maerz and Paul color blocks are enclosed in brackets. (Maen: and Paul, Dictionary of Color, McGraw-Hill, New York (1950)). Cultures were grown at 30 C. for 14 days unless otherwise noted.

TABLE Description of Culture NRRL 3601 Property Observed Characteristics of NRRL Morphology Flexuous sporophores were produced on fascicled aerial mycelium; the spores are cylindrical, measuring 0.67 to 1.0;; by 1.7 to 2.7 with smooth spore surfaces as observed in electron micro hs.

Cultural grap Characteristics on:

Oatmeal agar Inorganic salts and starch Growth moderate, reverse medium yellow [10H4]; aerial mycelium moderate, sponrlation fair, yellowish white (Y) Zba [9D2]; soluble pigment sparse brown; sclerotia present.

Glycerol- Asparagine agar Czapeks' agar Glucose-asparagine Tyrosine agar Nutrient agar Calcium malate Glycerol-glycine Tomato paste-oatmeal Emerson's agar Bennetts agar Action on milk liquefaction Temperature requirements Response of substrate color to pH change Growth moderate, reverse vivid yellow [9J5]; aerial mycelium and sporulation fair, white (W) a; no soluble pigment.

Growth abundant, reverse vivid yellow [1 U5]; aerial mycelium fair, white (W) a, no sporulation; no soluble pigment.

Growth abundant, reverse medium yellow l 1.16]; aerial mycelium abundant, sporulation fair, pale yellow (Y) Zba [9D2 no soluble pigment.

Growth moderate, reverse light grayish brown [l 182]; aerial mycelium moderate, white (W) a, sporulation scant, no soluble pigment; sclerotia present.

Growth moderate, reverse pale yellow green [IOBl aerial mycelium sparse, no sporulation; no soluble pigment; sclerotia present.

Growth abundant, reverse medium yellow [I015]; aerial mycelium and sponrlation moderate, pale orange yellow (R) 3m [9531; soluble pigment light yellow.

Growth abundant, reverse medium yellow [1 U6]; aerial mycelium moderate, sporulation fair, white (W) a; no soluble pigment.

Growth abundant, reverse medium orange to medium orange yellow [l0J7]; aerial mycelium and sporulation abundant, pale yellow (Y) 2120 [9D2]; no soluble pigment.

Growth abundant, reverse light yellow [1063], aerial mycelium sparse, no sporulation; no soluble pigment.

Growth abundant, reverse medium orange yellow [1016]; aerial mycelium abundant, sporulation fair, white (W) a; no soluble pigment.

Slight coagulation and some clearing at 14 days.

Slight reduction at 14 days.

Negative Negative Complete at l4 days.

Growth on yeast malt agar from 26 to 37"; no growth at 43.

Unaffected minus carbon (control) Utilization code:

+ positive utilization nun" probable utilization questionable utilization no utilization The culture medium employable in producing pyrazomycin by cultivating the above-described organism can be any one of several media, since, as is apparent from the above utilization tests, the organism is capable of utilizing different energy sources. However, for economy of production, maximum yield of antibion'c, and ease of isolation of the antibiotic, certain relatively simple nutrient sources are preferable. For example, the media which are useful in the production of the antibiotic include an assirnilable source of carbon such as dextrose, dextrine, glycerol, and the like. The presently preferred source of carbon is dextrose. Additionally, employable media include a source of assimilable nitrogen such as soybean meal, corn steep solids, yeast, cottonseed meal, beef extract, peptones (meat or soy), casein, amino acid mixtures and the like. Presently preferred sources of nitrogen are peptones, yeast extracts, amino acid mixtures, and the like. Among the nutrient inorganic salts which can be incorporated in the culture media are the customary salts capable of yielding sodium, potassium, ammonium, calcium, phosphate, sulfate, chloride, carbonate, and like ions.

Minor elements necessary for optimum growth and development of the organism used for the production of pyrazomycin should also be included in the culture medium. Such trace elements commonly occur as impurities in the other constituents of the medium in amounts suflicient to meet the growth requirements of the actinomycetes employed in this invention.

The initial pH of the culture medium can be varied. However, it has been found desirable that the initial pH of the medium be between about 6.0 to about 7.5. As has been observed with other actinomycetes, the pH of the medium gradually increases throughout the growth period of the organism while the antibiotic is being produced, and may attain a level of from about 6.5 to 8.0 or above, the final pH being dependent at least in part on the initial pH of the medium, the buflers present in the medium, and the period of time the organism is permitted to grow.

Submerged, aerobic cultural conditions are the conditions of choice for the production of pyrazomycin. For preparation of relatively small amounts, shake flask and surface culture in bottles can be employed; but for the preparation of large amounts, submerged aerobic culture in sterile tanks is preferred. The medium in the sterile tank can be inoculated with a sporulated suspension; but because of the growth lag experienced when a sporulated suspension is used as the inoculum, the vegetative form of the culture is preferred. By thus avoiding the growth lag, more efiicient use of the fermentation equipment is realized. Accordingly, it is desirable first to produce a vegetative inoculum of the organism by inoculating a relatively small quantity of culture medium with the spore form of the organism; and when a young, active vegetative inoculum has been obtained, to transfer the vegetative inoculum aseptically to the large tank. The medium in which the vegetative inoculum is produced can be either the same or different from the medium utilized for the large-scale production of pyrazomycin.

The organism which produces pyrazomycin will grow over a wide temperature range between 25-37 C. Optimal production of pyrazomycin seems to occur at temperatures of 25-30 C. In general, maximum production of the antibiotic occurs within about 48-72 hours after inoculation of the culture medium.

As is customary in aerobic, submerged culture processes, sterile air is blown through the culture medium. For eflicient growth of the organism and pyrazomycin production, the volume of air employed in the tank production of pyrazomycin is from 0.35 to 0.80 volume of air per minute per volume of culture. The preferred volume is 0.40 of air per minute per volume of culture medium.

The concentration of antibiotic activity in the culture medium can be followed readily during the fermentation period by testing samples of the culture medium for their activity against the growth of organisms known to be inhibited by the presence of pyrazomycin. The organism Neurospora spp. has been found useful for this purpose. The testing of the samples can be carried out by the well-known, cup-plate method.

In general, maximum production of pyrazomycin occurs within about two to three days after inoculation of the culture medium in submerged aerobic culture or shake flask culture processes.

The antibiotic activity produced during the fermentation of pyrazomycin can occur either in the antibiotic broth and/or in the mycelium. Accordingly, isolation techniques employed in the production of pyrazomycin are designed to permit maximum recovery of the antibiotic from either or both sources. Thus, for example, the fermentation broth as obtained may be filtered and the antibiotic recovered by adsorption from the filtrate using a suitable adsorbent, such as carbon, eluting the activity from the adsorbent with, for example, an acetonewater eluant. The crude pyrazomycin is further purified by ion exchange chromatography and gel filtration. In addition, the residual antibiotic present in the mycelial cake can be recovered by thorough extraction with a suitable solvent such as methanol. However, from 75-95 percent of the activity has been found in the broth.

In order to illustrate more fully the operation of the invention, the following examples are provided by way of illustration.

EXAMPLE 1 SHAKE FLASK PRODUCTION OF PYRAZOMYCIN Spores of Streptomyces candidus NRRL 3601 were inoculated on nutrient agar slants. The nutrient agar medium comprises g. of dextrin, 2 g. of an enzyme digested casein, l g. of beef extract, 1 g. of yeast extract, g. of agar and sufficient distilled water to make a total volume of 1 liter. The slants were inoculated with spores of Streptomyces candidus NRRL 3601 and incubated for four to 6 days at 30 C. The mature slant cultures were covered with sterile distilled water and gently scraped with a loop to loosen the spores and provide an aqueous suspension thereof. One milliliter of the resulting spore suspension was used to inoculate each 100 ml. portion of vegetative medium.

The vegetative culture medium was prepared by combining 15 g. of glucose, 15 g. of soybean meal, 5 g. of cornsteep solids, 2 g. of calcium carbonate, and 5 g. of sodium chloride with sufficient tap water to make the total volume 1 liter.

The vegetative inoculum was shaken for 48 hours at 30 C. on a reciprocal shaker having a two-inch stroke at 108 r.p.m. The inoculum so prepared was then utilized in the production of pyrazomycin as follows:

A production medium was prepared having the following composition:

Soybean meal 1 Casein NaNO,

Glucose syrup 2 CaCO,

Tap water One hundred milliliter portions of the production medium were placed in five hundred milliliter Erlenmeyer flasks, which were then sterilized at 120 C. for 30 minutes. When cooled, each flask was inoculated with a 5 percent vegetative inoculum prepared as previously described.

The production flasks were shaken for 48 hours at 30 C., on a rotary shaker having a 2-inch arc operating at 250 r.p.m. The pH of the uninoculated medium varied over a pH range of 6.0 to 7.5. The harvest pH at the end of the fermentation cycle was between 6.5 and 8.0. The antifungal and antiviral activity of pyrazomycin was found in both the broth and the mycelium. The activity was determined by assaying the broth and the mycelium against Neurospora spp. Isolation of pyrazomycin was carried out as described in Example 2.

EXAMPLE 2 PILOT PLANT PRODUCTION OF PYRAZOMYCIN The production of pyrazomycin by submerged fermentation on a pilot plant scale is illustrated by the following example.

To a 250 ml. flask containing 50 ml. of a vegetative medium comprising 10 g./l. of dextrose, 25 g./1. of dextrin, 20 g./l. of soy peptone, 5 g./l. of Nadrisol distillers solubles, and tap water, having a pH of 6.6, was added a lyophile pellet made from the mature nutrient agar slant cultures described in Example 1.

The inoculated vegetative medium was incubated at about 30 C. for 48 hours on a rotary shaker having a 2-inch diameter arc, operating at 250 r.p.m. The terminal pH of the medium was found to be 7.0. A 20 ml. portion of the vegetative culture so obtained was employed to inoculate a 1 liter flask containing 100 ml. of the vegetative medium. The inoculated vegetative medium was incubated at about 30 C. for 48 hours on a rotary shaker having a 2-inch diameter are, operating at 250 r.p.m. The terminal pH at the end of the second stage was 7.4. A 500 ml. portion of the vegetative culture as obtained was employed to inoculate a 40 literfermenter containing 25 liters of a sterilized aqueous fermentation medium comprising 0.2 g./l. of Dow Corning Antifoam A, 20 g./l. of glycerol, 5 g./l. of soy peptone, 2 g./l. of calcium nitrate, 0.5 g./l. of sodium chloride, 3 g./l. of Nadrisol distillers solubles, and tap water to bring the volume up to 25 liters. The fermentation was maintained at a temperature of 30 C. for 42 hours after inoculation. Agitation at the rate of 420 r.p.m. was carried out throughout the fermentation. Aeration throughout the fermentation was at a rate of 0.4 cubic feet of air per cubic foot of medium per minute. The terminal pH was 7.6.

The fermentation was harvested and the whole broth filtered. To 25 liters of filtered broth was added 10 percent (w./v.) of Pittsburg 12 X 40 mesh carbon. After stirring for 30 minutes, the mixture was filtered and washed with water. The activity was eluted from the carbon by three successive treatments with 9 liters of a 1:1 acetone-water solvent. The acetone-water eluates were combined and concentrated to a volume of about 500 ml. The concentrate was then passed through a 250 cc. Dowex 50W-X2 (H+ cycle) resin column, and the column washed with water. The activity is not exchanged onto the resin, and thus was collected in the effluent volume and subsequent water washes. The effluent and water washes were combined and the pH adjusted to approximately 7.0. The solution was then concentrated and applied to a 5.0 X cm. Sephadex G-10 dextran gel column in water. The column was eluted with water and the active fractions (assayed by disc plate assay of Neurospora spp. X-846) were combined, concentrated, and freeze-dried. Final purification was accomplished by column chromatography on an Avicel cellulose column equilibrated and eluted with 70 percent propanol. The freeze-dried active preparation from the Sephadex column was dissolved in a minimal amount of 70 percent propanol and applied to a 2.2 cm. X 60 cm. Avicel cellulose column. The active fractions were combined and concentrated to an aqueous solution and allowed to crystallize, to yield 850 mg. of pyrazomycin, m.p., l081 13 C., pKa 6.9

(water).

Analysis, Calc. for C,H,,-,N,O,: C, 41.70;

H, 5.00; N, 16.20; 0, 37.10. Found: C, 41.56;

We claim:

1. The antibiotic pyrazomycin, said antibiotic being a colorless crystalline solid substance melting at 108-1 13 C., which is soluble in water, methanol, ethanol, propanol, and butanol, is slightly soluble in ethyl acetate, and is insoluble in hydrocarbon solvents and ethyl ether; which has an optical rotation [a] of 45.5 (C 0.78 percent, w.v., water); which has the approximate composition of 41.56 percent carbon, 5.25 percent hydrogen; 16.02 percent nitrogen, and 36.25 percent oxygen; which has one titratable group of pK'a 6.9; which has a molecular weight, calculated from mass spectral data of 259; which has an empirical formula of C l-l N O which when measured as a potassium bromide pellet has the following distinguishable bands on its infrared absorption spectrum over the range of 2.0 to 15.0 microns as follows: 3.10; 3.40; 6.04; 6.21; 6.51; 6.80; 7.00; 8.50; 9.00; 9.30; 9.80; 11.20; 11.70; 12.90; 13.60; and 14.00 microns; and which is best represented by the structural formula HzN STATES PA'R'ENE URRHLE; v 111 1015 Patent No. 5,6715771? Dated 197 Inventor) Robert H Williams and Marvin M. Hoehn It is certified that error appears in the above-i&enti1e& patent and that said Letters Patent are hereby corrected as shown below:

In column 5, in Table I, the left hand. column should read as two columns asfollows:

conc. Zone mgc./ml. mm.

7' 22 1000 55 5- 5 20 500 55 1 5 I 55 1000 1 28 125 5 v 62 50 5 55 51 50 5 5 15 15 2 5O 1' 15 5 5 5 11 5 2 7' 12 5. 5 10 Signed and sealed this 20th day Of NOV 973- (SEAL) Attest:

EDWARD MFLETGHER ,JR. RENE D. TEGTMEYER Attesting Officer Acting Commissioner of Patents 

